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Text – The abstract should be: 250 words maximum, with title (bolded) and authors (presenter underlined), include sponsoring program(s) and institutional affiliation(s).
Assessing Surgical Tool Effect on Peripheral Nerve Function
Shonique White, Stephanie Brown*, & Dr. John Cavanaugh** Wayne State University – Summer Undergraduate Research Academy
University of Michigan, Western Michigan University*, Wayne State University**
New medical devices used for making and cauterizing surgical incisions make use of ultrasound (US) technology to complete the procedure. Utilizing high frequency vibrations, the instruments are able to cut and cauterize tissue. Heat is transferred to the tissue when these tools are used, but it is not as great as with traditional electrosurgery (ES) devices. The objective of this project was to determine whether US instruments operating in close proximity to peripheral nerves, has a detrimental effect, and to compare
this to effects using an ES device. To mock clinical procedures, small 10 mm incisions were made on either side of the sciatic nerve in anesthetized adult male Sprague-Dawley rats, 1 mm away and approximately 1-2 mm deep. To test the effect that the instrument use had on the nerve, electrical activity in the gastrocnemius, soleus, and tibialis anterior, three muscles the nerve innervates, was recorded. To keep the observed electrical activity constant, the nerve was stimulated with a hook electrode at 3, 5, and
7 volts, proximal to the incision. The electrical activity in the three muscles was recorded prior to surgery, and compared to the readings post surgery. If the electrical activity decreased in the post surgery readings from the baseline measurement, it suggests that the tool in question may have an adverse affect on the nerve function. Four instruments, three ultrasound devices and one electrosurgery device, were tested in this manner. The electrosurgery device made uses a monopolar blade, and the ultrasound devices include an ultrasound blade and ultrasound shears. In order to record the EMG data, Bortec and National Instruments data acquisition devices were used. Stimulation of the sciatic nerve was provided by A-M Systems’ Isolated Pulse Stimulator. To record the muscles’ response in EMG form, fine wire electrodes were inserted into the muscle belly and their signals were sent to the Bortec system for analysis. Sampling rates were taken at 5000 samples per second. Trial studies on expired rats were performed to develop the techniques described above. Experiments on live anesthetized rats approved under AIC protocol number
A 01-16-10 are still being carried out.
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